Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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Petroleum microbiology – Wikipedia

The results obtained from the identification were compared with Bergey Manual of Bacteriology Holt, The microbial utilization of such carbon-rich, but nitrogen-poor substrates necessitates the availability of nitrogenous compounds for the synthesis of cell materials such as proteins and nucleic acids.

Consortia can degrade naphthalene, fluorene, phenanthrene, anthracene, chrysene, fluoranthrene, benzopyrene, indenol and hundreds of polyaromatic hydrocarbons in shorter period. Bwcteria for Pseudomonas aeruginosa whose optimal growth was attained from the 10 th to 16 th day, the other four bacterial isolates reached stationary and death phases after the 8 th to 14 th day of degradation of the crude oil Table 2.

Typical gel electrophoresis bands of amplified nifH genes in the genomic DNA extract from the bacterial species tested. Kuwait was suitable for this study because it is a small approximately18, km 2but major oil-producing country. Pseudomonas stutzeri partial nifH gene for putative nitrogenase iron protein, strain Gr Eighty-two out of the hydrocarbonoclastic bacterial species tested were capable of growing on the NaNO 3 -free mineral medium with oil bafteria the sole source of carbon and energy.

Microbiology of Extreme Soils. Weaker peaks indicate more effective consumption. Bioremediation, Hydrocarbonoclastic, Bacteria, Refinery Effluent. Materials and Methods Bacterial isolates One hundred different species of oil-utilizing bacteria that have been deposited in our private culture collection were used in this study.


Quantitative studies on marine biodegradation of oil. Soil bacteria mentioned above are also found in freshwater as they are carried by rainwater runoff, water drainage or via discharge of effluents. These species had been isolated from various pristine and oil-polluted Gulf habitats, including desert soil, the rhizospheres and phyllospheres of wild and cultivated plants, seawater, hypersaline areas, and air dust, on a mineral medium 24 with oil vapor as the sole source of carbon and energy.

Obligate marine hydrocarbon bacteria genera include Marinobacter, Marinobacterium, Alcanivorax, Neptunomonas, Thalassolituus, Cycloclasticus, Oleispira and Oleiphilus.

The toxicity of hydrocarbon can result in cancer, gastrointestinal infection, heart disease, neurological disorder and respiratory infection Leachy and Rita, Bacterial biosensor systems offer the potential for cheap, robust detection systems that are selective and highly sensitive. Environ Sci Pollut Res. HCB are important constituents of oil degrading consortia and are usually present in hydrocarbon contaminated sites.

The two general strategies for enhancing oil recovery are altering the surface properties of the interface and using bioclogging to change the flow behavior. By using this site, you agree to the Terms of Use and Privacy Policy. The medium used in this test contained 0.

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Journal List Microbes Environ v. They utilize hydrocarbons as carbon and energy source for growth. Hydrocarbonoclastic bacteria isolated from petroleum hydrocarbbonoclastic sites in Tunisia: Hydrocarbonoclastic microorganisms are involved in the mineralization of hydrocarbon pollutants in the total environment.

Sequencing of gel bands I and II in Fig. Developments in Biodeterioration of Hydrocarbons.

Partial 16S rRNA gene sequencing performed on 50 isolates showed high level of identity with known sequences. The highest emulsification activity was detected in Pseudomonas geniculata with IPPW-3 nitrogenase iron protein nifH gene. Although, maximum efficacy were observed using gram positive bacteria which is contrary to our report of gram negative bacteria. The five bacteria isolates with hydrocarbonoclastic potentials were isolated and identified as Pseudomonas aeruginosa, Proteus hydrocarbonolasticKlebsiella pneumoniae, Serretia marcescens and Streptomyces sp.

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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Orphaned articles from April All orphaned articles. These 15 bacterial species tested positive for nitrogenase, as revealed by the GLC-detection of ethylene peaks in their culture top space gases GLC profiles not shown. Enhancement of rhizosphere of crop plants raised in oily bacterix with hydrocarbon-utilizing bacteria capable of hydrocarbon consumption in nitrogen free media.

Contact and attachment to the hydrocarbon molecule is thought to be pre-requisite step for oil degradation. It constitutes the bacreria mechanism for the elimination of hydrocarbons from contaminated sites by natural existing populations of microorganisms.

Potential Hydrocarbonoclastic Bacteria

Hydrocarbon degradation in soil is therefore influenced by their availability for uptake by HCB. Utilization of aliphatic hydrocarbons by microorganisms.

Regarding isolations from sea water and hypersaline areas, 0. Hydrocarbons are compounds whose structures are made up of hydrogen and carbon atoms.

The dominant groups of bacteria isolated from KRPC drains were gram negative bacteria for possible hydrocarbon degradation S.

There are 74 active users right now. Join as an Editorial Member. Biodegradation of crude oil by nitrogen fixation marine bacteria Azotobacter chrococcum. Author information Article notes Copyright and License information Disclaimer. An amount of 0. Most 12 species of these species belonged to Alphaproteobacteria and Bxcteriawhich was consistent with previous findings on hydrocarbonoclastic bacteria in contaminated sediments and seawater from a refinery harbor in the north of Tunisia All articles on this website are for general information only and is not a professional or experts advice.

Achromobacter, Actinobacter, Alcaligenes, Arthrobacter, Flavobacterium, Nocardia and Bacteris species are also hydrocarbon degraders Leachy et al.

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